Modular Design of Self-Assembling Peptide-Based Nanotubes
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- 作者：Natasha C. Burgess ; Thomas H. Sharp ; Franziska Thomas ; Christopher W. Wood ; Andrew R. Thomson ; Nathan R. Zaccai ; R. Leo Brady ; Louise C. Serpell ; Derek N. Woolfson
- 刊名：Journal of the American Chemical Society
- 出版年：2015
- 出版时间：August 26, 2015
- 年：2015
- 卷：137
- 期：33
- 页码：10554-10562
- 全文大小：845K
- ISSN：1520-5126
文摘
An ability to design peptide-based nanotubes (PNTs) rationally with defined and mutable internal channels would advance understanding of peptide self-assembly, and present new biomaterials for nanotechnology and medicine. PNTs have been made from Fmoc dipeptides, cyclic peptides, and lock-washer helical bundles. Here we show that blunt-ended 伪-helical barrels, that is, preassembled bundles of 伪-helices with central channels, can be used as building blocks for PNTs. This approach is general and systematic, and uses a set of de novo helical bundles as standards. One of these bundles, a hexameric 伪-helical barrel, assembles into highly ordered PNTs, for which we have determined a structure by combining cryo-transmission electron microscopy, X-ray fiber diffraction, and model building. The structure reveals that the overall symmetry of the peptide module plays a critical role in ripening and ordering of the supramolecular assembly. PNTs based on pentameric, hexameric, and heptameric 伪-helical barrels sequester hydrophobic dye within their lumens.