Imparting Antifouling Properties of Poly(2-hydroxyethyl methacrylate) Hydrogels by Grafting Poly(oligoethylene glycol methyl ether acrylate)

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• 作者：Dimitriya Bozukova ; Christophe Pagnoulle ; Marie-Claire De Pauw-Gillet ; Nadia Ruth ; Robert J&#xe9 ; rô ; me ; Christine J&#xe9 ; rô ; me
• 刊名：Langmuir
• 出版年：2008
• 出版时间：July 1, 2008
• 年：2008
• 卷：24
• 期：13
• 页码：6649 - 6658
• 全文大小：939K
• 年卷期：v.24,no.13(July 1, 2008)
• ISSN：1520-5827

文摘

The antifouling properties of poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) hydrogels were improved by the surface grafting of a brush of poly(oligoethylene glycol methyl ether acrylate) [poly(OEGA)]. The atom-transfer radical polymerization (ATRP) of OEGA (degree of polymerization = 8) was initiated from the preactivated surface of the hydrogel under mild conditions, thus in water at 25 °C. The catalytic system was optimized on the basis of two ligands [1,1,4,7,10,10-hexamethyl-triethylenetetramine (HMTETA) or tris[2-(dimethylamino)ethyl]amine (Me₆TREN)] and two copper salts (Cu^IBr or Cu^ICl). Faster polymerization was observed for the Me₆TREN/Cu^IBr combination. The chemical composition and morphology of the coated surface were analyzed by X-ray photoelectron spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy, contact angle measurements by the water droplet and captive bubble methods, scanning electron microscopy, and environmental scanning electron microscopy. The hydrophilicity of the surface increased with the molar mass of the grafted poly(OEGA) chains, and the surface modifications were reported in parallel. The antifouling properties of the coatings were tested by in vitro protein adsorption and cell adhesion tests, with green fluorescent protein, β-lactamase, and lens epithelial cells, as model proteins and model cells, respectively. The grafted poly(OEGA) brush decreased the nonspecific protein adsorption and imparted high cell repellency to the hydrogel surface.