Determination of Six Pyrazole Fungicides in Grape Wine by Solid-Phase Extraction and Gas Chromatography–Tandem Mass Spectrometry

详细信息    查看全文

• 作者：Yan Shen ; Zhou Li ; Qiang Ma ; Chuanxian Wang ; Xiangzhun Chen ; Qian Miao ; Chao Han
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：May 18, 2016
• 年：2016
• 卷：64
• 期：19
• 页码：3901-3907
• 全文大小：342K
• 年卷期：0
• ISSN：1520-5118

文摘

A gas chromatography–tandem mass spectrometry (GC–MS/MS) method was developed for the first simultaneous identification and quantification of six pyrazole fungicides (furametpyr, rabenzazole, fluxapyroxad, penflufen, bixafen, and isopyrazam) in grape wine samples. The grape wine samples were first diluted with water, then purified by solid-phase extraction, and finally examined by GC–MS/MS in multiple reaction monitoring (MRM) mode. Matrix-matched calibration curves were used to correct the matrix effects. The limits of quantification (LOQs), calculated as 10 times the standard deviation, were 0.2–0.8 μg kg^–1 for the six pyrazole fungicides. The average recoveries were in the range of 74.3–94.5%, with relative standard deviations (RSDs) below 5.8%, measured at three concentration levels. The proposed method is suitable for the simultaneous determination of six pyrazole fungicides in grape wine samples.