Interaction between PEO鈥揚PO鈥揚EO Copolymers and a Hexapeptide in Aqueous Solutions

详细信息    查看全文

• 作者：Lianwei Jia ; Chen Guo ; Junfeng Xiang ; Ning Wang ; Liangrong Yang ; Yalin Tang ; Huizhou Liu
• 刊名：Langmuir
• 出版年：2012
• 出版时间：January 24, 2012
• 年：2012
• 卷：28
• 期：3
• 页码：1725-1732
• 全文大小：458K
• 年卷期：v.28,no.3(January 24, 2012)
• ISSN：1520-5827

文摘

Interaction between PEO鈥揚PO鈥揚EO copolymers and a hexapeptide, growth hormone releasing peptide-6 (GHRP-6), was investigated by NMR to study the potential use of the copolymers in peptide drug delivery. ¹H NMR and nuclear Overhauser effect spectroscopy (NOESY) measurements determined that PO methyl protons interacted with methyl protons of the Ala moiety, aromatic protons of the Trp moiety, and some of the Phe aromatic protons. The Lys moiety and part of the Phe moiety entered the hydrophilic EO environment via hydrogen bonding. PEO鈥揚PO鈥揚EO copolymers and the peptide formed a complex in 1:1 stoichiometry. Binding constants between copolymers and GHRP-6 were determined, and it was indicated that the copolymers containing more EO and PO units will lead to greater affinity with the peptide. Isothermal titration calorimetry (ITC) measurements confirmed the results of NMR experiments. This study indicates that PEO鈥揚PO鈥揚EO copolymers have great potential in delivering peptide drugs.