Two-Input Fluorescent Probe for Thiols and Hydrogen Sulfide Chemosensing and Live Cell Imaging

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• 作者：Chun-Guang Dai ; Xiu-Ling Liu ; Xiao-Jiao Du ; Yan Zhang ; Qin-Hua Song
• 刊名：ACS Sensor
• 出版年：2016
• 出版时间：July 22, 2016
• 年：2016
• 卷：1
• 期：7
• 页码：888-895
• 全文大小：638K
• ISSN：2379-3694

文摘

Development of multi-input fluorescent probes would facilitate the monitoring of multiple biomolecular events leading to a common disease pathology. Here, we reported a two-input fluorescent probe (QME-N₃) based on quinoline scaffold with two distinct reactive sites, the α,β-unsaturated carbonyl at 2-site and 6-azido group, which were specific acceptors of RSH and H₂S respectively. The turn-on fluorescent probe could react with the two molecules via the Michael addition at the double bond for RSH and the reduction of the azide by H₂S without mutual interference, to generate the intensively fluorescent product, which could act as a two-input AND logic gate. Furthermore, QME-N₃ has very low cytotoxicity and excellent stability, and the resulting sensing product has a high two-photon absorption cross section. Consequently, detection of intracellular thiols and H₂S can be achieved by cell fluorescence imaging under both one- and two-photon excitations.