Intracellular Delivery of a Membrane-Impermeable Enzyme in Active Form Using Functionalized Gold Nanoparticles

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• 作者：Partha Ghosh ; Xiaochao Yang ; Rochelle Arvizo ; Zheng-Jiang Zhu ; Sarit S. Agasti ; Zhihong Mo ; Vincent M. Rotello
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：March 3, 2010
• 年：2010
• 卷：132
• 期：8
• 页码：2642-2645
• 全文大小：253K
• 年卷期：v.132,no.8(March 3, 2010)
• ISSN：1520-5126

文摘

Gold nanoparticles were coated with a short peptide to promote intracellular delivery of membrane-impermeable proteins. Through microscopy and enzyme assays, we demonstrated the particles were able to transport functional enzymes into a variety of cell lines. Significantly, the transported proteins were able to escape from endosomes. Moreover, these particles showed no apparent cytotoxicity.