Enhanced Detection and Characterization of Glutathione-Trapped Reactive Metabolites by Pseudo-MS3 Transition Using a Linear Ion Trap Mass Spectrometer
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- 作者：Xiaochun Zhu ; Mike Hayashi ; Raju Subramanian
- 刊名：Chemical Research in Toxicology
- 出版年：2012
- 出版时间：September 17, 2012
- 年：2012
- 卷：25
- 期：9
- 页码：1839-1841
- 全文大小：219K
- 年卷期：v.25,no.9(September 17, 2012)
- ISSN：1520-5010
文摘
We present a simple and label-free approach to characterize glutathione (GSH)-trapped reactive metabolites from a single LC-MS analysis employing a linear ion trap mass spectrometer. The GSH specific fragment anion m/z 272 was first generated from the nonselective in-source fragmentation of intact conjugates. GSH conjugates were then detected by selected reaction monitoring (SRM) of the anion pair m/z 272 鈫?179 or 210. The resultant SRM peaks represented putative GSH conjugates which were then further characterized from their MS and MS2 data acquired in both positive and negative ion modes. The method is demonstrated with test compounds that are all known to form GSH conjugates.