Polyspermine imine, a pH Responsive Polycationic siRNA Carrier Degradable to Endogenous Metabolites
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- 作者：Zixiu Du ; Shengnan Xiang ; Yi Zang ; Yi Zhou ; Chuandong Wang ; Hailing Tang ; Tuo Jin ; Xiaoling Zhang
- 刊名：Molecular Pharmaceutics
- 出版年：2014
- 出版时间：October 6, 2014
- 年：2014
- 卷：11
- 期：10
- 页码：3300-3306
- 全文大小：362K
- ISSN：1543-8392
文摘
Cationic polymers readily degradable in response to cellular environment are especially favored as easy-formulating materials to pack siRNA into a nanoparticle and to release the cargo in the cytoplasm in time. In addition to the efficiency of cytosomal release, the degradation products should best be free of safety concerns, a typical challenge for cationic polymers. To satisfy the two criteria, we report a new cationic polymer, polyspermine imine, named as PSP-Imine, which is formed by condensing two endogenous molecules, spermine and glyoxal, through conjugated 蟺 linkage, 鈥擭鈺怌鈥擟鈺怤鈥?(Schiff base reaction), a poly linkage structure sufficiently stable under neutral condition but dissociative under the endosomal pH. Cellular assays under a confocal microscope indicated that the polyplex formed of PSP-Imine readily released the loaded siRNA to the cytoplasm after being engulfed in the target cells and efficiently silenced the target genes in different cell lines and xenograft mouse model of human cervical carcinoma, as compared with nondegradable PEI 25 kDa. Cell viability assays confirmed that PSP-Imine showed no visible cytotoxicity within the concentration being tested. The present study suggests that PSP-Imine is an excellent siRNA condensing material for forming the core of a therapeutically feasible synthetic carrier system.