pH-Controlled Construction of Chitosan/Alginate Multilayer Film: Characterization and Application for Antibody Immobilization
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- 作者：Weiyong Yuan ; Hua Dong ; Chang Ming Li ; Xiaoqiang Cui ; Ling Yu ; Zhisong Lu ; Qin Zhou
- 刊名：Langmuir
- 出版年：2007
- 出版时间：December 18, 2007
- 年：2007
- 卷：23
- 期：26
- 页码：13046 - 13052
- 全文大小：378K
- 年卷期：v.23,no.26(December 18, 2007)
- ISSN：1520-5827
文摘
In this work, a chitosan/alginate multilayer film was constructed via layer-by-layer self-assembly and studied byin situ surface plasmon resonance monitoring and contact angle measurements. The results demonstrate that the surfacecomposition of the self-assembled multilayer film can be simply tailored through pH control during the assemblyprocess. The biological property of the assembled film was further characterized via antigen-antibody interactions,showing that the loading capacity of the antibody on the multilayered film and the binding activity of the antigen tothe immobilized antibody could be also well-tuned by pH control. This work can provide more scientific insight inthe interaction between protein and polymer matrix and render a novel simple approach to build high-performancebiointerfaces through pH control for potential applications of highly sensitive immunosensors.