文摘
This paper reports the successful isolation and characterization of a novel phenol-degrading bacterial strain WUST-C1 from activated sludge. WUST-C1 is an aerobic, Gram-negative, nonsporulating, and short-rod or peanut-shaped bacterium, which occurs singly, in pairs, but mainly in clusters. 16S rDNA sequence alignment demonstrated this strain belonged to the genus of pseudomonas, with a 99.9% identity to 16S rDNA sequences of Pseudomonas aeruginosa EU170480. It could mineralize up to 1200 mg L鈥? phenol within 36 h (99%). Besides phenol, WUST-C1 could grow aerobically on pyrocatechol, alpha-naphthol, hydroquinone, naphthalene, isoquinoline, and indole. WUST-C1 was resistant to ampicillin and chloromycetin. The growth kinetics of strain WUST-C1 on phenol as a sole carbon and energy source at 35 掳C can be described using Haldane equation. It has a maximum specific growth rate (渭max) of 2.47 h鈥?, a half-saturation constant (Ks) of 48.7 mg L鈥?, and a substrate inhibition constant (Ki) of 100.6 mg L鈥? with a R2-value of 0.988. Strain WUST-C1 is tolerant to phenol at concentrations up to 1600 mg L鈥?. Hence, it could potentially be an excellent bacterial candidate for the biotreatment of high-strength phenol-containing industrial wastewaters and for the in situ bioremediation of phenol-contaminated sites.