Isolation and Characterization of a Novel Phenol Degrading Bacterial Strain WUST-C1

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• 作者：Jianzhong Liu ; Qian Wang ; Jiabao Yan ; Xiaorong Qin ; Lingling Li ; Wu Xu ; Ramalingam Subramaniam ; Rakesh K. Bajpai
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2013
• 出版时间：January 9, 2013
• 年：2013
• 卷：52
• 期：1
• 页码：258-265
• 全文大小：360K
• 年卷期：v.52,no.1(January 9, 2013)
• ISSN：1520-5045

文摘

This paper reports the successful isolation and characterization of a novel phenol-degrading bacterial strain WUST-C1 from activated sludge. WUST-C1 is an aerobic, Gram-negative, nonsporulating, and short-rod or peanut-shaped bacterium, which occurs singly, in pairs, but mainly in clusters. 16S rDNA sequence alignment demonstrated this strain belonged to the genus of pseudomonas, with a 99.9% identity to 16S rDNA sequences of Pseudomonas aeruginosa EU170480. It could mineralize up to 1200 mg L^鈥? phenol within 36 h (99%). Besides phenol, WUST-C1 could grow aerobically on pyrocatechol, alpha-naphthol, hydroquinone, naphthalene, isoquinoline, and indole. WUST-C1 was resistant to ampicillin and chloromycetin. The growth kinetics of strain WUST-C1 on phenol as a sole carbon and energy source at 35 掳C can be described using Haldane equation. It has a maximum specific growth rate (渭_max) of 2.47 h^鈥?, a half-saturation constant (K_s) of 48.7 mg L^鈥?, and a substrate inhibition constant (K_i) of 100.6 mg L^鈥? with a R²-value of 0.988. Strain WUST-C1 is tolerant to phenol at concentrations up to 1600 mg L^鈥?. Hence, it could potentially be an excellent bacterial candidate for the biotreatment of high-strength phenol-containing industrial wastewaters and for the in situ bioremediation of phenol-contaminated sites.