A novel method for the purification of crude polysaccharide from fermentation broth of pumpkin residues by macroporous resins was developed. Through static adsorption
and desorption
and adsorption kinetic tests, six resins (AB-8, S-8, HPH480, HPD100, X-5,
and D101) with different polarity, diameter,
and surface area were studied for simultaneous decoloration
and deproteinization of crude polysaccharide,
and S-8 was chosen as the best one. Dynamic breakthrough
and desorption tests were performed in a glass column packed with S-8 resin,
and the resulting adsorption ratios of pigment
and protein were 84.3%
and 75.9% (w/w), respectively, with a recovery ratio of polysaccharide 84.7% (w/w). S-8 resin also exhibited higher purification efficiency than the other tested traditional methods. Moreover, UV/vis spectroscopy (200鈥?00 nm) analysis revealed most of the pigment
and protein were absorbed by S-8 resin,
and HPLC (containing a refractive index detector
and a HPSEC column) results indicated that there was no degradation of the polysaccharide. This automated
and efficient method via adsorption鈥揹esorption strategy could have potential in scale-up purification
and preparation of polysaccharide in the future.
Keywords:
purification; adsorption; decoloration; deproteinization; polysaccharide; macroporous resin