Simultaneous Decoloration and Deproteinization of Crude Polysaccharide from Pumpkin Residues by Cross-Linked Polystyrene Macroporous Resin
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- 作者：Rui Yang ; Demei Meng ; Yi Song ; Juan Li ; Yuyu Zhang ; Xiaosong Hu ; Yuanying Ni ; Quanhong Li
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：August 29, 2012
- 年：2012
- 卷：60
- 期：34
- 页码：8450-8456
- 全文大小：409K
- 年卷期：v.60,no.34(August 29, 2012)
- ISSN：1520-5118
文摘
A novel method for the purification of crude polysaccharide from fermentation broth of pumpkin residues by macroporous resins was developed. Through static adsorption and desorption and adsorption kinetic tests, six resins (AB-8, S-8, HPH480, HPD100, X-5, and D101) with different polarity, diameter, and surface area were studied for simultaneous decoloration and deproteinization of crude polysaccharide, and S-8 was chosen as the best one. Dynamic breakthrough and desorption tests were performed in a glass column packed with S-8 resin, and the resulting adsorption ratios of pigment and protein were 84.3% and 75.9% (w/w), respectively, with a recovery ratio of polysaccharide 84.7% (w/w). S-8 resin also exhibited higher purification efficiency than the other tested traditional methods. Moreover, UV/vis spectroscopy (200鈥?00 nm) analysis revealed most of the pigment and protein were absorbed by S-8 resin, and HPLC (containing a refractive index detector and a HPSEC column) results indicated that there was no degradation of the polysaccharide. This automated and efficient method via adsorption鈥揹esorption strategy could have potential in scale-up purification and preparation of polysaccharide in the future.