Fabrication of Lignosulfonate Vesicular Reverse Micelles to Immobilize Horseradish Peroxidase
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- 作者：Xiaowen Zhong ; Yong Qian ; Jinhao Huang ; Dongjie Yang ; Yonghong Deng ; Xueqing Qiu
- 刊名：Industrial & Engineering Chemistry Research
- 出版年：2016
- 出版时间：March 16, 2016
- 年：2016
- 卷：55
- 期：10
- 页码：2731-2737
- 全文大小：435K
- ISSN：1520-5045
文摘
Sodium lignosulfonate reverse micelles (SLRMs) with vesicular structure were prepared by self-assembling in ethanol–water media and applied to encapsulate horseradish peroxidase (HRP). Results showed that sodium lignosulfonate (SL) could not form SLRMs until the ethanol content reached 63% when its initial concentration was 7.5 g L–1. Owing to strong electrostatic repulsion, solid spherical SLRMs gradually swelled to stable vesicular structures with an average size of 240 nm. The shell of the SLRM thickened when NaCl was added to screen the electrostatic interaction. HRP can be effectively encapsulated while retaining its activity in the hydrophilic core of a SLRM. When hydrogen peroxide was added to initiate the catalytic activity of HRP, SL molecules would be polymerized and the structure of SLRMs would be fixed. Furthermore, HRP immobilized in polymerized SLRMs showed high activity at a more acidic pH of 4 and at a lower optimal temperature decrease of 35 °C compared to free HRP. SLRM allows enzymes such as HRP to work at more acidic and lower temperature conditions.