Europium Nanospheres-Based Time-Resolved Fluorescence for Rapid and Ultrasensitive Determination of Total Aflatoxin in Feed

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• 作者：Du Wang ; Zhaowei Zhang ; Peiwu Li ; Qi Zhang ; Xiaoxia Ding ; Wen Zhang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：December 2, 2015
• 年：2015
• 卷：63
• 期：47
• 页码：10313-10318
• 全文大小：296K
• ISSN：1520-5118

文摘

Immunochromatographic (IC) assays are considered suitable diagnostic tools for the determination of mycotoxins. A europium nanospheres-based time-resolved fluorescence immunoassay (Eu-Nano-TRFIA), based on a monoclonal antibody and a portable TRFIA reader, was developed to determine total aflatoxin (including aflatoxins B₁, B₂, G₁, and G₂) levels in feed samples. Under optimized conditions, the Eu-Nano-TRFIA method detected total aflatoxin within 12 min. It showed good linearity (R² > 0.985), LOD of 0.16 渭g/kg, a wide dynamic range of 0.48鈥?0.0 渭g/kg, recovery rates of 83.9鈥?13.9%, and coefficients of variation (CVs) of 3.5鈥?.8%. In the 397 samples from company and livestock farms throughout China, the detection rate was 78.3%, concentrations were 0.50鈥?45.30 渭g/kg, the highest total aflatoxin content was found in cottonseed meal, and corn was found to be the most commonly contaminated feed. This method could be a powerful alternative for the rapid and ultrasensitive determination of total aflatoxin in quality control and meet the required Chinese maximum residue limits.