Luminmycins A鈥揅, Cryptic Natural Products from Photorhabdus luminescens Identified by Heterologous Expression in Escherichia coli
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- 作者：Xiaoying Bian ; Alberto Plaza ; Youming Zhang ; Rolf M眉ller
- 刊名：Journal of Natural Products
- 出版年：2012
- 出版时间：September 28, 2012
- 年：2012
- 卷：75
- 期：9
- 页码：1652-1655
- 全文大小：206K
- 年卷期：v.75,no.9(September 28, 2012)
- ISSN：1520-6025
文摘
The 18 kb 鈥渟ilent鈥?luminmycin biosynthetic pathway from Photorhabdus luminescens was cloned into a vector by using the newly established linear鈥搇inear homologous recombination and successfully expressed in Escherichia coli. Luminmycins A鈥揅 (1鈥?b>3) were isolated from the heterologous host, and their structures were elucidated using 2D NMR spectroscopy and HRESIMS. Luminmycin A is a deoxy derivative of the previously reported glidobactin A, while luminmycins B and C most likely represent its acyclic biosynthetic intermediates. Compound 1 showed cytotoxicity against the human colon carcinoma HCT-116 cell line with an IC50 value of 91.8 nM, while acyclic 2 was inactive at concentrations as high as 100 渭g/mL.