Hydrogen Sulfide Detection Based on Reflection: From a Poison Test Approach of Ancient China to Single-Cell Accurate Localization
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- 作者：Hao Kong ; Zhuoran Ma ; Song Wang ; Xiaoyun Gong ; Sichun Zhang ; Xinrong Zhang
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：August 5, 2014
- 年：2014
- 卷：86
- 期：15
- 页码：7734-7739
- 全文大小：361K
- ISSN：1520-6882
文摘
With the inspiration of an ancient Chinese poison test approach, we report a rapid hydrogen sulfide detection strategy in specific areas of live cells using silver needles with good spatial resolution of 2 脳 2 渭m2. Besides the accurate-localization ability, this reflection-based strategy also has attractive merits of convenience and robust response when free pretreatment and short detection time are concerned. The success of endogenous H2S level evaluation in cellular cytoplasm and nuclear of human A549 cells promises the application potential of our strategy in scientific research and medical diagnosis.