Design of Ratiometric Emission Probe with Visible Light Excitation for Determination of Ca2+ in Living Cells

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• 作者：Qiaoling Liu ; Huizhi Du ; Xiaoze Ren ; Wei Bian ; Li Fan ; Shaomin Shuang ; Chuan Dong ; Qin Hu ; Martin M. F. Choi
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：August 19, 2014
• 年：2014
• 卷：86
• 期：16
• 页码：8025-8030
• 全文大小：316K
• ISSN：1520-6882

文摘

An organic salt as a fluorescent probe based on intramolecular charge transfer for Ca²⁺ determination is developed. Ca²⁺ can be detected by ratiometric emission at 490 and 594 nm with an excitation wavelength of 405 nm. This probe is highly selective for Ca²⁺ over other divalent metal cations and displays a large Stokes shift of 189 nm that can avoid interference of the excitation light beam and autofluorescence of biological samples. The dissociation constant for Ca²⁺ is 2.25 卤 0.47 渭M and pertinent to Ca²⁺ detection in cellular resting and dynamic states. The probe demonstrates its application in monitoring Ca²⁺ in living cells under confocal microscopic imaging.