Attachment of Marine Sponge Cells of Hymeniacidon perleve on Microcarriers

详细信息    查看全文

• 作者：Quanyu Zhao ; Meifang Jin ; Werner E. G. M&uuml ; ller ; Wei Zhang ; Xingju Yu ; and Maicun Deng
• 刊名：Biotechnology Progress
• 出版年：2003
• 出版时间：October 2003
• 年：2003
• 卷：19
• 期：5
• 页码：1569 - 1573
• 全文大小：108K
• 年卷期：v.19,no.5(October 2003)
• ISSN：1520-6033

文摘

Toward the development of an in vitro cultivation of marine sponge cells for sustainableproduction of bioactive metabolites, the attachment characteristics of marine spongecells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, andglass beads, were studied. Mixed cell population and enriched cell fractions of specificcell types by Ficoll gradient centrifugation (6%/8%/15%/20%) were also assessed. Cellattachment ratio (defined as the ratio of cells attached on microcarrier to the totalnumber of cells in the culture) on glass beads is much higher than that on Cytodex 3and Hillex for both mixed cell population and cell fraction at Ficoll 15-20% interface.The highest attachment ratio of 41% was obtained for the cell fraction at Ficoll 15-20% interface on glass beads, which was significantly higher than that of a mixed cellpopulation (18%). The attachment kinetics on glass beads indicated that the attachment was completed within 1 h. Cell attachment ratio decreases with increase in cell-to-microcarrier ratio (3-30 cells/bead) and pH (7.6-9.0). The addition of serum andBSA (bovine serum albumin) reduced the cell attachment on glass beads.