Agarose-Based Microfluidic Device for Point-of-Care Concentration and Detection of Pathogen

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• 作者：Yiwei Li ; Xinghua Yan ; Xiaojun Feng ; Jie Wang ; Wei Du ; Yachao Wang ; Peng Chen ; Liang Xiong ; Bi-Feng Liu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：November 4, 2014
• 年：2014
• 卷：86
• 期：21
• 页码：10653-10659
• 全文大小：399K
• ISSN：1520-6882

文摘

Preconcentration of pathogens from patient samples represents a great challenge in point-of-care (POC) diagnostics. Here, a low-cost, rapid, and portable agarose-based microfluidic device was developed to concentrate biological fluid from micro- to picoliter volume. The microfluidic concentrator consisted of a glass slide simply covered by an agarose layer with a binary tree-shaped microchannel, in which pathogens could be concentrated at the end of the microchannel due to the capillary effect and the strong water permeability of the agarose gel. The fluorescent Escherichia coli strain OP50 was used to demonstrate the capacity of the agarose-based device. Results showed that 90% recovery efficiency could be achieved with a million-fold volume reduction from 400 渭L to 400 pL. For concentration of 1 脳 10³ cells mL^鈥? bacteria, approximately ten million-fold enrichment in cell density was realized with volume reduction from 100 渭L to 1.6 pL. Urine and blood plasma samples were further tested to validate the developed method. In conjugation with fluorescence immunoassay, we successfully applied the method to the concentration and detection of infectious Staphylococcus aureus in clinics. The agarose-based microfluidic concentrator provided an efficient approach for POC detection of pathogens.