Infrared Spectral Analysis of MCF-7 Cells Treated with Serum-Lipid Extracts Segregates Predominantly Brominated Flame Retardant-Exposed Subjects from Those with Mainly Organochlorine Exposures
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- 作者：Xinhui Bi ; Michael J. Walsh ; Xiaomin Wei ; Guoying Sheng ; Jiamo Fu ; Pierre L. Martin-Hirsch ; Gareth O. Thomas ; Kevin C. Jones ; Francis L. Martin
- 刊名：Environmental Science & Technology
- 出版年：2007
- 出版时间：August 15, 2007
- 年：2007
- 卷：41
- 期：16
- 页码：5915 - 5922
- 全文大小：304K
- 年卷期：v.41,no.16(August 15, 2007)
- ISSN：1520-5851
文摘
We set out to determine whether infrared (IR) spectra oftreated MCF-7 cells derived using attenuated totalreflection Fourier-transform IR (ATR) microspectroscopycould be used to signature different exposures. Serum-lipid samples from residents living in an electronics waste (e-waste) dismantling region (Region E, n = 26) in thesouth of P.R. China or from a nearby region (Region S, n= 20) associated with the fishing industry were obtained.Following extraction, estrogen receptor-positive MCF-7cells were treated for 24 h with 
5-mg lipid equivalent ofindividual extracts prior to ethanol fixation; subsequently,IR spectra of treated-cell populations were obtained usingATR microspectroscopy. Levels of organochlorinated andbrominated compounds in parallel extracts were alsodetermined. Principal component analysis (PCA) wasemployed to ascertain whether IR-spectral scores wouldcluster according to particular cohort. Scores plots followingPCA highlighted significant clustering dependent on withwhich set of extracts MCF-7 cells were treated. This suggestsmarked differences in the cell-biochemical modulatingeffects induced by either set of extracts. Analysis ofcontaminant profiles in the extracts showed that polybrominated diphenyl ethers (PBDEs) accounted for 46.0%of total organohalogens and were higher than dichloro-diphenyl-trichloroethanes (DDTs) in Region E; DDTs werethe major contaminants (85.2% of total contaminant load)compared to PBDEs (8.7%) in Region S. These results suggestthat ATR microspectroscopy can segregate cell-biochemicaleffects as a consequence of very different exposureparadigms.
5-mg lipid equivalent ofindividual extracts prior to ethanol fixation; subsequently,IR spectra of treated-cell populations were obtained usingATR microspectroscopy. Levels of organochlorinated andbrominated compounds in parallel extracts were alsodetermined. Principal component analysis (PCA) wasemployed to ascertain whether IR-spectral scores wouldcluster according to particular cohort. Scores plots followingPCA highlighted significant clustering dependent on withwhich set of extracts MCF-7 cells were treated. This suggestsmarked differences in the cell-biochemical modulatingeffects induced by either set of extracts. Analysis ofcontaminant profiles in the extracts showed that polybrominated diphenyl ethers (PBDEs) accounted for 46.0%of total organohalogens and were higher than dichloro-diphenyl-trichloroethanes (DDTs) in Region E; DDTs werethe major contaminants (85.2% of total contaminant load)compared to PBDEs (8.7%) in Region S. These results suggestthat ATR microspectroscopy can segregate cell-biochemicaleffects as a consequence of very different exposureparadigms.