Interfacial Bioorthogonal Cross-Linking

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• 作者：Han Zhang ; Kevin T. Dicker ; Xian Xu ; Xinqiao Jia ; Joseph M. Fox
• 刊名：ACS Macro Letters
• 出版年：2014
• 出版时间：August 19, 2014
• 年：2014
• 卷：3
• 期：8
• 页码：727-731
• 全文大小：464K
• ISSN：2161-1653

文摘

Described herein is interfacial bioorthogonal cross-linking, the use of bioorthogonal chemistry to create and pattern biomaterials through diffusion-controlled gelation at the liquid-gel interface. The basis is a rapid (k₂ 284000 M^鈥? s^鈥?) reaction between strained trans-cyclooctene (TCO) and tetrazine (Tz) derivatives. Syringe delivery of Tz-functionalized hyaluronic acid (HA-Tz) to a bath of bis-TCO cross-linker instantly creates microspheres with a cross-linked shell through which bis-TCO diffuses freely to introduce further cross-linking at the interface. Tags can be introduced with 3D resolution without external triggers or templates. Water-filled hydrogel channels were prepared by simply reversing the order of addition. Prostate cancer cells encapsulated in the microspheres have 99% viability, proliferate readily, and form aggregated clusters. This process is projected to be useful in the fabrication of cell-instructive matrices for in vitro tissue models.