Black Tea Increased Survival of Caenorhabditis elegans under Stress
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- 作者：Li-Gui Xiong ; Jian-An Huang ; Juan Li ; Peng-Hui Yu ; Zhe Xiong ; Jian-Wei Zhang ; Yu-Shun Gong ; Zhong-Hua Liu ; Jin-Hua Chen
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：November 19, 2014
- 年：2014
- 卷：62
- 期：46
- 页码：11163-11169
- 全文大小：403K
- ISSN：1520-5118
文摘
The present study examined the effects of black tea (Camellia sinensis) extracts (BTE) in Caenorhabditis elegans under various abiotic stressors. Results showed BTE increased nematode resistance to osmosis, heat, and UV irradiation treatments. However, BTE could not increase nematodes鈥?lifespan under normal culture conditions and MnCl2-induced toxicity at concentrations we used. Further studies showed that BTE decreased reactive oxygen species and up-regulated some antioxidant enzymes, including GSH-PX, and genes, such as gsh-px and sod-3. However, only a slight extension in mev-1 mutants mean lifespan was observed without significance. These results indicated that the antioxidant activity of BTE might be necessary but not sufficient to protect against aging to C. elegans. Moreover, BTE increased the mRNA level of stress-response genes such as sir-2.1 and sek-1. Our finding demonstrated BTE might increase heat and UV stress resistance in a sir.2.1-dependent manner. Taken together, BTE enhanced stress resistance with multiple mechanisms in C. elegans.