Development of an Immunochromatographic Lateral-Flow Test Strip for Rapid Detection of Sulfonamides in Eggs and Chicken Muscles

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• 作者：Xiliang Wang ; Kui Li ; Deshi Shi ; Ning Xiong ; Xiue Jin ; Jundong Yi ; Dingren Bi
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2007
• 出版时间：March 21, 2007
• 年：2007
• 卷：55
• 期：6
• 页码：2072 - 2078
• 全文大小：153K
• 年卷期：v.55,no.6(March 21, 2007)
• ISSN：1520-5118

文摘

A rapid immunochromatographic lateral-flow test strip was developed in the competitive reaction formatfor the detection of sulfonamides in eggs and chicken muscle. A monoclonal antibody against thecommon structure of sulfonamides was conjugated to colloidal gold particles as the detection reagentand an N-sulfanilyl-4-aminobenzoic acid (SUL)-bovine serum albumin (BSA) conjugate wasimmobilized to a nitrocellulose membrane as the capture reagent to prepare the test strip. With thismethod, it required only 15 min to accomplish the semiquantitative or quantitative detection ofsulfonamides. The sensitivity to sulfonamides (sulfamonomethoxine, sulfamethoxydiazine, sulfadimethoxine, and sulfadiazine) was at least 10 ng/mL, as determined with an optical density scanner.By eye measurement, the sensitivity was 20 ng/mL for sulfamonomethoxine, sulfamethoxydiazine,and sulfadimethoxine and 40 ng/mL for sulfadiazine. On the basis of a sulfamonomethoxine standardcurve, recoveries were from 89.5 to 95.6% for sulfamonomethoxine, from 89.5 to 95.1% forsulfamethoxydiazine, from 85.0 to 95.6% for sulfadimethoxine, and from 44.8 to 60.9% for sulfadiazinein egg and chicken muscle samples. A parallel analysis of 27 egg samples and 28 chicken musclesamples from the animal experiment showed that the differences between test strips and high-performance liquid chromatography (HPLC) were from 0.8 to 11.2% for egg samples and from 2.2 to34% for chicken muscle samples for the quantitative detection, and the agreement rates betweentest strips and HPLC were 100%, based on the maximum allowed residue level of sulfadiazine (100ng/g) established by the European Union and China. In conclusion, the method is rapid and accuratefor the detection of sulfonamides in eggs and chicken muscles.