Novel Fe3O4@TiO2 Core−Shell Microspheres for Selective Enrichment of Phosphopeptides in Phosphoproteome Analysis

详细信息    查看全文

• 作者：Yan Li ; Xiuqing Xu ; Dawei Qi ; Chunhui Deng ; Pengyuan Yang ; Xiangmin Zhang
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：7
• 期：6
• 页码：2526 - 2538
• 全文大小：2940K
• 年卷期：v.7,no.6(June 2008)
• ISSN：1535-3907

文摘

Due to the dynamic nature and low stoichiometry of protein phosphorylation, enrichment of phosphorylated peptides from proteolytic mixtures is often necessary prior to their characterization by mass spectrometry. Immobilized metal affinity chromatography (IMAC) is a popular way to enrich phosphopeptides; however, conventional IMAC lacks enough specificity for efficient phosphoproteome analysis. In this study, novel Fe₃O₄@TiO₂ microspheres with well-defined core−shell structure were prepared and developed for highly specific purification of phosphopeptides from complex peptide mixtures. The enrichment conditions were optimized using tryptic digests of β-casein, and the high specificity of the Fe₃O₄@TiO₂ core−shell microspheres was demonstrated by effectively enriching phosphopeptides from the digest mixture of α-casein and β-casein, as well as a five-protein mixture containing nonphosphoproteins (bovine serum albumin (BSA), myoglobin, cytochrome c) and phosphoproteins (ovalbumin and β-casein). The Fe₃O₄@TiO₂ core−shell microspheres were further successfully applied for the nano-LC−MS/MS analysis of rat liver phosphoproteome, which resulted in identification of 56 phosphopeptides (65 phosphorylation sites) in mouse liver lysate in a single run, indicating the excellent performance of the Fe₃O₄@TiO₂ core−shell microspheres.