Chemical-Induced pH-Mediated Molecular Switch

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• 作者：Dilani A. Jayawardhana ; Mrinal K. Sengupta ; D.M. Milan Krishantha ; Jyoti Gupta ; Daniel W. Armstrong ; Xiyun Guan
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：October 15, 2011
• 年：2011
• 卷：83
• 期：20
• 页码：7692-7697
• 全文大小：817K
• 年卷期：v.83,no.20(October 15, 2011)
• ISSN：1520-6882

文摘

The transmembrane protein 伪-hemolysin pore has been used to develop ultrasensitive biosensors, study biomolecular folding and unfolding, investigate covalent and noncovalent bonding interactions, and probe enzyme kinetics. Here, we report that, by addition of ionic liquid tetrakis(hydroxymethyl)phosphonium chloride solution to the 伪-hemolysin pore, the 伪-hemolysin channel can be controlled open or closed by adjusting the pH of the solution. This approach can be employed to develop a novel molecular switch to regulate molecular transport and should find potential applications as a 鈥渟mart鈥?drug delivery method.