Loop-Mediated Isothermal Amplification Integrated on Microfluidic Chips for Point-of-Care Quantitative Detection of Pathogens

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• 作者：Xueen Fang ; Yingyi Liu ; Jilie Kong ; Xingyu Jiang
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：April 1, 2010
• 年：2010
• 卷：82
• 期：7
• 页码：3002-3006
• 全文大小：203K
• 年卷期：v.82,no.7(April 1, 2010)
• ISSN：1520-6882

文摘

This work shows that loop-mediated isothermal amplification (LAMP) of nucleic acid can be integrated in an eight-channel microfluidic chip for readout either by the naked eye (as a result of the insoluble byproduct pyrophosphate generating during LAMP amplification) or via absorbance measured by an optic sensor; we call this system microLAMP (μLAMP). It is capable of analyzing target nucleic acids quantitatively with high sensitivity and specificity. The assay is straightforward in manipulation. It requires a sample volume of 0.4 μL and is complete within 1 h. The sensitivity of the assay is comparable to standard methods, where 10 fg of DNA sample could be detected under isothermal conditions (63 °C). A real time quantitative μLAMP assay using absorbance detection is possible by integration of optical fibers within the chip.