Predicting Viruses Accurately by a Multiplex Microfluidic Loop-Mediated Isothermal Amplification Chip

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• 作者：Xueen Fang ; Hui Chen ; Shaoning Yu ; Xingyu Jiang ; Jilie Kong
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：February 1, 2011
• 年：2011
• 卷：83
• 期：3
• 页码：690-695
• 全文大小：848K
• 年卷期：v.83,no.3(February 1, 2011)
• ISSN：1520-6882

文摘

Multiplex gene assay is a valuable molecular tool not only in academic science but also in clinical diagnostics. Multiplex PCR assays, DNA microarrays, and various nanotechnology-based methods are examples of major techniques developed for analyzing multiple genes; none of these, however, are suitable for point-of-care diagnostics, especially in resource-limited settings. In this report, we describe an octopus-like multiplex microfluidic loop-mediated isothermal amplification (m渭LAMP) assay for the rapid analysis of multiple genes in the point-of-care format and provide a robust approach for predicting viruses. This assay with the ability of analyzing multiple genes qualitatively and quantitatively is highly specific, operationally simple, and cost/time-effective with the detection limit of less than 10 copies/渭L in 2 渭L quantities of sample within 0.5 h. We successfully developed a m渭LAMP chip for differentiating three human influenza A substrains and identifying eight important swine viruses.