Nanoparticles Comprising a Mixed Monolayer for Specific Bindings with Biomolecules

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• 作者：Ming Zheng and Xueying Huang
• 刊名：Journal of the American Chemical Society
• 出版年：2004
• 出版时间：September 29, 2004
• 年：2004
• 卷：126
• 期：38
• 页码：12047 - 12054
• 全文大小：172K
• 年卷期：v.126,no.38(September 29, 2004)
• ISSN：1520-5126

文摘

This work presents a strategy of using mixed monolayer protected nanoparticles for specificinteractions with target biological molecules. The mixed monolayer is composed of a shielding componentand a capture component. The shielding component utilizes ethylene glycol oligomers to prevent nonspecificbinding with biomolecules. The capture component is chosen to specifically interact with the target of interest,such as a protein molecule. Such a concept was demonstrated by two synthetic systems. The first one isgold nanoparticles protected by a mixed monolayer of tri(ethylene glycol) thiol (EG₃-SH) and tiopronin(Tp), which was prepared by a one-step synthesis. Surface chemical composition studies using ¹H NMRspectroscopy revealed that the reactivity of EG₃-SH is 3 times as high as that of Tp in the nanoparticleformation. Gel electrophoresis analysis identified a critical ratio of (EG₃-S-)/Tp on the nanoparticle surfaceabove which no nonspecific binding occurred. By further derivatizing Tp into a biotin group, we synthesizedAu(-S-EG₃)_n/Tp-biotin particles that bind specifically to streptavidin with negligible nonspecific binding.The second system is gold nanoparticles protected by a mixed monolayer of EG₃-SH and glutathione(GSH). By controlling the feeding ratio of EG₃-SH and GSH, we made Au(-S-EG₃)_n/GSH particles thatbind specifically to gultathione-S-transferase (GST) with negligible nonspecific binding.