We report a size-exclusion chromatography (SEC) processto purify DNA-wrapped carbon nanotubes (DNA-CNT) andto sort them into fractions of uniform length. A type ofsilica-based column resin was identified that showsminimum adsorption of DNA-CNT. Three such columnsin series with pore sizes of 2000, 1000, and 300 Å werefound to separate DNA-CNT into fractions of very narrowlength distribution, as measured directly by atomic forcemicroscopy. The average length decreases monotonicallyfrom >500 nm in the early fractions to <100 nm in thelate fractions, with length variation
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10% in each of themeasured fractions. Using UV-vis-NIR spectroscopy, weshowed that SEC is very effective in removing graphiticimpurities that contribute to the spectral baseline and abroad absorption peak at ~270 nm. This result highlightsthe importance of CNT purification in the study of opticalproperties of CNT.