In
spi
red
by the availa
bility of
de novo t
ran
sc
riptome of ho
rse g
ram (
Macrotyloma uniflorum) and
recent development
s in
sy
stem
s biology
studie
s, the fi
rst eve
r glo
bal p
rotein–p
rotein inte
ractome (PPI) map wa
s con
st
ructed fo
r thi
s highly d
rought-tole
rant legume. La
rge-
scale
studie
s of PPI
s and the con
st
ructed data
ba
se would p
rovide
rationale
behind the inte
rplay at ca
scading t
ran
slational level
s fo
r d
rought
st
re
ss-adaptive mechani
sm
s in ho
rse g
ram. U
sing a
bidi
rectional app
roach (inte
rolog and domain-
ba
sed), a high-confidence inte
ractome map and data
ba
se fo
r ho
rse g
ram wa
s con
st
ructed. Availa
ble t
ran
sc
riptomic info
rmation fo
r shoot and
root ti
ssue
s of a
sen
sitive (M-191; genotype 1) and a d
rought-tole
rant (M-249; genotype 2) genotype of ho
rse g
ram wa
s utilized to d
raw compa
rative PPI
su
bnetwo
rk
s unde
r d
rought
st
re
ss. High-confidence 6804 inte
raction
s we
re p
redicted among 1812 p
rotein
s cove
ring a
bout one-fou
rth of the ho
rse g
ram p
roteome. The highe
st num
be
r of inte
raction
s (33.86%) in ho
rse g
ram inte
ractome matched with
Arabidopsis PPI data. The top five hu
b node
s mo
stly included u
biquitin and heat-
shock-
related p
rotein
s. Highe
r num
be
rs of PPI
s we
re found to
be
re
spon
sive in
shoot ti
ssue (416) and
root ti
ssue (2228) of genotype 2 compa
red with
shoot ti
ssue (136) and
root ti
ssue (579) of genotype 1. Cha
racte
rization of PPI
s u
sing gene ontology analy
si
s revealed that kina
se and t
ran
sfe
ra
se activitie
s involved in
signal t
ran
sduction, cellula
r p
roce
sse
s, nucleocytopla
smic t
ran
spo
rt, p
rotein u
biquitination, and localization of molecule
s we
re mo
st
re
spon
sive to d
rought
st
re
ss. Hence, the
se could
be f
ramed in
st
re
ss adaptive mechani
sm
s of ho
rse g
ram. Being the fi
rst legume glo
bal PPI map, it would p
rovide new in
sight
s into gene and p
rotein
regulato
ry netwo
rk
s fo
r d
rought
st
re
ss tole
rance mechani
sm
s in ho
rse g
ram. Info
rmation compiled in the fo
rm of data
ba
se (
MauPIR) will p
rovide the much needed high-confidence
sy
stem
s biology info
rmation fo
r ho
rse g
ram gene
s, p
rotein
s, and involved p
roce
sse
s. Thi
s info
rmation would ea
se the effo
rt and inc
rea
se the efficacy fo
r simila
r studie
s on othe
r legume
s. Pu
blic acce
ss i
s availa
ble at
ref="http://14.139.59.221/MauPIR/" class="extLink">http://14.139.59.221/MauPIR/.