Competitive Binding Assay for Glucose Based on Glycodendrimer-Fluorophore Conjugates

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• 作者：Bennett L. Ibey ; Hope T. Beier ; Rebecca M. Rounds ; Gerard L. Coté ; Vamsi K. Yadavalli ; and Michael V. Pishko
• 刊名：Analytical Chemistry
• 出版年：2005
• 出版时间：November 1, 2005
• 年：2005
• 卷：77
• 期：21
• 页码：7039 - 7046
• 全文大小：375K
• 年卷期：v.77,no.21(November 1, 2005)
• ISSN：1520-6882

文摘

A new fluorescent glucose assay has been created usingAlexa Fluor 647-labeled concanavalin A (Con A) and afourth-generation PAMAM Alexa Fluor 594-labeled glycodendrimer. This assay has been shown to have a largeresponse to glucose within the biological range and to becapable of functioning within a polymer hydrogel. In thispaper, the glucose response is shown to be a singlefluorophore-based quenching reaction. Data showing thatthe sensor is fully reversible and specific through competitive binding between the dendrimer and glucose withCon A are presented. Overall, the assay is shown to havepotential over the traditional dextran-based assay becauseit has a larger dynamic response to physiological glucoseconcentrations, incorporates longer wavelength dyes thatimprove signal penetration through dermal tissue, andprovides an internal reference in the form of a nonreactivefluorescent label.