Biotinylation of Silicon and Nickel Surfaces and Detection of Streptavidin as Biosensor

详细信息    查看全文

• 作者：Hirokazu Seto ; Chie Yamashita ; Seiji Kamba ; Takashi Kondo ; Makoto Hasegawa ; Mitsuhiro Matsuno ; Yuichi Ogawa ; Yu Hoshino ; Yoshiko Miura
• 刊名：Langmuir
• 出版年：2013
• 出版时间：July 30, 2013
• 年：2013
• 卷：29
• 期：30
• 页码：9457-9463
• 全文大小：360K
• 年卷期：v.29,no.30(July 30, 2013)
• ISSN：1520-5827

文摘

The availability of metal mesh device sensors has been investigated using surface-modified nickel mesh. Biotin was immobilized on the sensor surfaces consisting of silicon and nickel via a thiol鈥揺ne click reaction, known as the Michael addition reaction. Biotinylation on the maleimidated surface was confirmed by X-ray photoelectron spectroscopy. The binding of streptavidin to the biotinylated surfaces was evaluated using a quartz crystal microbalance and a metal mesh device sensor, with both techniques providing similar binding constant value. The recognition ability of the biotin immobilized using the thiol-maleimide method for streptavidin was comparable to that of biotin immobilized via several other methods. The adsorption of a biotin conjugate onto the streptavidin-immobilized surface via the biotin鈥搒treptavidin鈥揵iotin sandwich method was evaluated using a fluorescent microarray, with the results demonstrating that the biological activity of the streptavidin remained.