Engineering the Autotroph Methanococcus maripaludis for Geraniol Production

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• 作者：Zhe Lyu ; Rachit Jain ; Peyton Smith ; Travis Fetchko ; Yajun Yan ; William B. Whitman
• 刊名：ACS Synthetic Biology
• 出版年：2016
• 出版时间：July 15, 2016
• 年：2016
• 卷：5
• 期：7
• 页码：577-581
• 全文大小：295K
• 年卷期：0
• ISSN：2161-5063

文摘

The rapid autotrophic growth of the methanogenic archaeon Methanococcus maripaludis on H₂ and CO₂ makes it an attractive microbial chassis to inexpensively produce biochemicals. To explore this potential, a synthetic gene encoding geraniol synthase (GES) derived from Ocimum basilicum was cloned into a M. maripaludis expression vector under selection for puromycin resistance. Recombinant expression of GES in M. maripaludis during autotrophic growth on H₂/CO₂ or formate yielded geraniol at 2.8 and 4.0 mg g^–1 of dry weight, respectively. The yield of geraniol decreased 2–3-fold when organic carbon sources were added to stimulate heterotrophic growth. In the absence of puromycin, geraniol production during autotrophic growth on formate increased to 4.6 mg g^–1 of dry weight. A conceptual model centered on the autotrophic acetyl coenzyme A biosynthetic pathway identified strategies to divert more autotrophic carbon flux to geraniol production.