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• 作者：Zhuhai Chen ; Yang Liu ; Yangzhong Wang ; Xin Zhao ; Jinghong Li
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：May 7, 2013
• 年：2013
• 卷：85
• 期：9
• 页码：4431-4438
• 全文大小：459K
• 年卷期：v.85,no.9(May 7, 2013)
• ISSN：1520-6882

文摘

A sandwich electrogenerated chemiluminescence (ECL) biosensor was fabricated based on concanavalin A (Con A)-integrating gold-nanoparticle-modified Ru(bpy)₃²⁺-doped silica nanoprobe (Au鈥揜uSiO₂ NPs) for in situ and dynamically evaluating cell surface N-glycan expression. Owing to the specific recognition of Con A with mannose and the core trimannoside fragment of N-glycan and the effective ECL amplification of Au鈥揜uSiO₂ NPs, the as-proposed biosensor exhibited excellent analytical performance toward the cytosensing of K562 cells with a wide detection linear range from 1.0 脳 10³ to 1.0 脳 10⁷ cells mL^鈥? and a detection limit of 600 cells mL^鈥?. More importantly, the strategy was successfully applied to evaluate cell surface N-glycan expression under different external stimuli of inhibitors and enzyme. This biosensor is endowed with feasibility and reliability of generating sensitive insight into the majority of N-glycan expression on the cell surface. Furthermore, the biosensor was employed to dynamically profile cell surface N-glycan expression at different phases of cell growth in vitro. This biosensor is promising in studying and elucidating the N-glycan function in biological and physiological processes.