Surface Functionalization of TiO2 Nanotubes with Bone Morphogenetic Protein 2 and Its Synergistic Effect on the Differentiation of Mesenchymal Stem Cells

详细信息    查看全文

• 作者：Min Lai ; Kaiyong Cai ; Li Zhao ; Xiuyong Chen ; Yanhua Hou ; Zaixiang Yang
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：April 11, 2011
• 年：2011
• 卷：12
• 期：4
• 页码：1097-1105
• 全文大小：1154K
• 年卷期：v.12,no.4(April 11, 2011)
• ISSN：1526-4602

文摘

To investigate the influence of surface-functionalized substrates with nanostructures on the behaviors of mesenchymal stem cells, we conjugated bone morphogenetic protein 2 (BMP2) onto TiO₂ nanotubes with different diameter sizes of 30, 60, and 100 nm for in vitro study. Polydopamine was employed as the intermediate layer for the conjugation of BMP2. The successful conjugation of BMP2 onto TiO₂ nanotubes was revealed by field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and contact angle measurements. Immunofluorescence staining of vinculin, osteocalcin (OCN), and osteopontin (OPN) revealed that BMP2-functionalized TiO₂ nanotubes was favorable for cell growth. More importantly, MSCs cultured onto BMP2-functionalized TiO₂ nanotubes displayed significantly higher (p < 0.05 or p < 0.01) differentiation levels of ALP and mineralization after 7 and 14 day cultures, respectively. The results suggested that surface functionalization of TiO₂ nanotubes with BMP2 was beneficial for cell proliferation and differentiation. The approach presented here has potential application for the development of titanium-based implants for enhanced bone osseointegration.