Simultaneous Analysis of Tertiary Butylhydroquinone and 2-tert-Butyl-1,4-benzoquinone in Edible Oils by Normal-Phase High-Performance Liquid Chromatography
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- 作者：Jun Li ; Yanlan Bi ; Wei Liu ; Shangde Sun
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2015
- 出版时间：September 30, 2015
- 年：2015
- 卷：63
- 期：38
- 页码：8584-8591
- 全文大小：320K
- ISSN：1520-5118
文摘
During the process of antioxidation of tertiary butylhydroquinone (TBHQ) in oil and fat systems, 2-tert-butyl-1,4-benzoquinone (TQ) can be formed. The toxicity of TQ was much more than that of TBHQ. In the work, a normal-phase high-performance liquid chromatography (NP-HPLC) method for the accurate and simultaneous detection of TBHQ and TQ in edible oils was investigated. A C18 column was used to separate TBHQ and TQ, and the gradient elution solutions consisted of n-hexane containing 5% ethyl acetate and n-hexane containing 5% isopropanol. The ultraviolet (UV) detector was set at dual wavelength mode (280 nm for TBHQ and 310 nm for TQ). The column temperature was 30 掳C. Before the NP-HPLC analysis, TBHQ and TQ were first extracted by methanol, subjected to vortex treatment, and then filtered through a 0.45 渭m membrane filter. Results showed that linear ranges of TBHQ and TQ were both within 0.10鈥?00.00 渭g/mL (R2 > 0.9999). The limit of detection (LOD) and limit of quantification (LOQ) of TBHQ and TQ were below 0.30 and 0.91 渭g/mL and below 0.10 and 0.30 渭g/mL, respectively. The recoveries of TBHQ and TQ were 98.92鈥?02.34 and 96.28鈥?00.58% for soybean oil and 96.11鈥?9.42 and 98.83鈥?9.24% for lard, respectively. These results showed that NP-HPLC can be successfully used to analyze simultaneously TBHQ and TQ in the oils and fats.