Identification of Glycopeptides with Multiple Hydroxylysine O-Glycosylation Sites by Tandem Mass Spectrometry

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• 作者：Yanlin Zhang ; Chuan-Yih Yu ; Ehwang Song ; Shuai Cheng Li ; Yehia Mechref ; Haixu Tang ; Xiaowen Liu
• 刊名：Journal of Proteome Research
• 出版年：2015
• 出版时间：December 4, 2015
• 年：2015
• 卷：14
• 期：12
• 页码：5099-5108
• 全文大小：524K
• ISSN：1535-3907

文摘

Glycosylation is one of the most common post-translational modifications in proteins, existing in 鈭?0% of mammalian proteins. Several research groups have demonstrated that mass spectrometry is an efficient technique for glycopeptide identification; however, this problem is still challenging because of the enormous diversity of glycan structures and the microheterogeneity of glycans. In addition, a glycopeptide may contain multiple glycosylation sites, making the problem complex. Current software tools often fail to identify glycopeptides with multiple glycosylation sites, and hence we present GlycoMID, a graph-based spectral alignment algorithm that can identify glycopeptides with multiple hydroxylysine O-glycosylation sites by tandem mass spectra. GlycoMID was tested on mass spectrometry data sets of the bovine collagen 伪-(II) chain protein, and experimental results showed that it identified more glycopeptide-spectrum matches than other existing tools, including many glycopeptides with two glycosylation sites.