文摘
Flos Chrysanthemi (Gongju, GJ) is used to prepare a herbal tea that is commonly consumed as a health beverage in Asia and is believed to contain abundant beneficial antioxidants. To rapidly identify the chemical constituents and to obtain the profile related to antioxidant activity, an online analytical method combining high-performance liquid chromatography鈥揹iode-array detector鈥揺lectrospray ionization鈥搃on-trap time-of-flight mass spectrometry (HPLC-DAD-ESI-IT-TOF-MSn) and postcolumn derivatization (PCD) has been applied for a precise and thorough identification of the chemical constituents. Meanwhile, the antioxidant profile has also been characterized by directly measuring the scavenging activity of each compound for the free radical produced by DPPH. As a result, 13 compounds have been identified in GJ, 7 of which account for its antioxidant activity. The established LC-MSn-PCD system has proved to offer a useful strategy for correlating the chemical profile with the bioactivities of the components without their isolation and purification, and may be used for multicomponent analysis of active substances in other foods and herbs.
Keywords:
Flos Chrysanthemi (Gongju); HPLC-DAD-ESI-IT-TOF-MSn coupled with postcolumn derivatization (PCD); online identification; antioxidant constituents