We used a modified yeast-based human estrogen receptor
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(ER
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) bioassay to determine theestrogenic activity in 22 kinds of diets for experimental animals. The estrogenic activity of each dietwas reevaluated by comparison with a calibration curve of 17
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-estradiol. Almost all of the diets hadestrogenic activity. The diets for rabbits and guinea pigs had the highest estrogenic activity comparedto any other diets, including those for rats and mice. Estrogenic activity was found in dried skim milk,fishmeal, soybean meal, and alfalfa meal. In the NIH-07 diet opened for the ingredients, estrogenicactivity was nearly all derived from the alfalfa meal. Multiple assays were performed to evaluatepotential seasonal variations in the estrogenic potency in the raw materials of the rat and mousediets. We found that the estrogenic activity in these raw materials changed throughout the year.Keywords: Diets; estrogenic activity; in vitro assay; genistein; daizein; coumesterol; alfalfa; soybean