Localized Surface Plasmon Resonance Detection of Biological Toxins Using Cell Surface Oligosaccharides on Glyco Chips
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- 作者：Takehiro Nagatsuka ; Hirotaka Uzawa ; Keita Sato ; Satoshi Kondo ; Masayuki Izumi ; Kenji Yokoyama ; Isaac Ohsawa ; Yasuo Seto ; Paola Neri ; Hiroshi Mori ; Yoshihiro Nishida ; Masato Saito ; Eiichi Tamiya
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2013
- 出版时间：May 22, 2013
- 年：2013
- 卷：5
- 期：10
- 页码：4173-4180
- 全文大小：557K
- 年卷期：v.5,no.10(May 22, 2013)
- ISSN：1944-8252
文摘
We have detected biological toxins using localized surface plasmon resonance (LSPR) and synthetic glycosyl ceramides (尾-lactoside, globosyl trisaccharide (Gb3), or GM1 pentasaccharide) attached to gold (Au) nanoparticles. The particle diameters ranged from 5鈥?00 nm. The detection sensitivity for three toxins (ricin, Shiga toxin, and cholera toxin) was found to depend not only on the attached glycoside but also on the diameter of the Au nanoparticles. For the detection of ricin, the 20-nm 尾-lactoside-coated Au nanoparticle exhibited the highest LSPR response, whereas 40-nm Gb3- and GM1-coated Au nanoparticles gave the best results for Shiga toxin and cholera toxin, respectively. In addition, a blocking process on the nanoparticle surface greatly improved the detection sensitivity for cholera toxin. The LSPR system enabled us to detect ricin at 30 ng/mL, Shiga toxin at 10 ng/mL, and the cholera toxin at 20 ng/mL.