Biopolymer鈥揕ipid Bilayer Interaction Modulates the Physical Properties of Liposomes: Mechanism and Structure

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• 作者：Chen Tan ; Yating Zhang ; Shabbar Abbas ; Biao Feng ; Xiaoming Zhang ; Wenshui Xia ; Shuqin Xia
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 19, 2015
• 年：2015
• 卷：63
• 期：32
• 页码：7277-7285
• 全文大小：604K
• ISSN：1520-5118

文摘

This study was conducted to elucidate the conformational dependence of the modulating ability of chitosan, a positively charged biopolymer, on a new type of liposome composed of mixed lipids including egg yolk phosphatidylcholine (EYPC) and nonionic surfactant (Tween 80). Analysis of the dynamic and structure of bilayer membrane upon interaction with chitosan by fluorescence and electron paramagnetic resonance techniques demonstrated that, in addition to providing a physical barrier for the membrane surface, the adsorption of chitosan extended and crimped chains rigidified the lipid membrane. However, the decrease in relative microviscosity and order parameter suggested that the presence of chitosan coils disturbed the membrane organization. It was also noted that the increase of fluidity in the lipid bilayer center was not pronounced, indicating the shallow penetration of coils into the hydrophobic interior of bilayer. Microscopic observations revealed that chitosan adsorption not only affected the morphology of liposomes but also modulated the particle aggregation and fusion. Especially, a number of very heterogeneous particles were visualized, which tended to confirm the role of chitosan coils as a 鈥減olymeric surfactant鈥? In addition to particle deformation, the membrane permeability was also tuned. These findings may provide a new perspective to understand the physiological functionality of biopolymer and design biopolymer鈥搇iposome composite structures as delivery systems for bioactive components.