Target-Cell-Specific Fluorescence Silica Nanoprobes for Imaging and Theranostics of Cancer Cells

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• 作者：Henan Li ; Yawen Mu ; Jusheng Lu ; Wei Wei ; Yakun Wan ; Songqin Liu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：April 1, 2014
• 年：2014
• 卷：86
• 期：7
• 页码：3602-3609
• 全文大小：406K
• 年卷期：v.86,no.7(April 1, 2014)
• ISSN：1520-6882

文摘

MicroRNAs (miRNAs) has been identified as diagnostic and prognostic biomarkers and predictors of drug response for many diseases, including a broad range of cancers, heart disease, and neurological diseases. The noninvasive theranostics system for miRNAs is very important for diagnosis and therapy of the cellular disease. Herein, a target-cell-specific theranostics nanoprobe for target-cell-specific delivery, cancer cells and intracellular miRNA-21 imaging, and cancer cell growth inhibition was proposed. The nanoprobe (FS-AS/MB) was prepared by simultaneously coupling of the AS1411 aptamer and miRNA-21 molecular beacon (miR-21-MB) onto the surface of Ru(bpy)₃²⁺-encapsulated silica (FS) nanoparticles. The FS nanoparticles synthesized by a facile reverse microemulsion method showed nearly monodisperse spherical shape with a smooth surface, good colloidal stability, a fluorescence quantum yield of 21%, and low cytotoxicity. The antibiofouling polymer PEG grafted onto a silica shell reduced nonspecific uptake of cells. The ability of FS-AS/MB for target-specific cells delivery, simultaneous cancer cells, intracellular miRNA-21 imaging, and inhibition of miRNA-21 function and suppression of cell growth in vitro, were also demonstrated. The results of the present study suggested that the proposed nanoprobes would be a promising theranostics for different cancers by imaging and inhibiting other intracellular genes.