Genetically Engineered Plant Viral Nanoparticles Direct Neural Cells Differentiation and Orientation

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• 作者：Sheng Feng ; Lin Lu ; Xingjie Zan ; Yehong Wu ; Yuan Lin ; Qian Wang
• 刊名：Langmuir
• 出版年：2015
• 出版时间：September 1, 2015
• 年：2015
• 卷：31
• 期：34
• 页码：9402-9409
• 全文大小：563K
• ISSN：1520-5827

文摘

An important aim of tissue engineering is to design biomimetic materials with specific cell binding motifs and precisely controlled structural organization, thereby providing biochemical and physical cues for desired cellular behaviors. Previously, our group generated genetically modified tobacco mosaic virus (TMV) displaying integrin binding motifs, RGD1, RGD7, PSHRN3, P15, and DGEA. The resulting rod-like virus particles displaying integrin binding motifs were biocompatible with Neuro 2A (N2a), a mouse neural crest-derived cell line, and could promote the neurite outgrowth of N2a. The genetically modified viruses could be assembled with aligned orientation in the capillary by applying a shear force. The resulting aligned substrates were able to dictate directional neurite outgrowth of N2a cells. Therefore, this method could be potentially applied for neural tissue engineering, as a neural conduit for repairing peripheral nerve injuries.