Determination of Niclosamide Residues in Rainbow Trout (Oncorhynchus mykiss) and Channel Catfish (Ictalurus punctatus) Fillet Tissue by High-Performance Liquid Chromatography
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- 作者：Theresa M. Schreier ; Verdel K. Dawson ; Yeong Choi ; Nancy J. Spanjers ; and Michael A. Boogaard
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2000
- 出版时间：June 2000
- 年：2000
- 卷：48
- 期：6
- 页码：2212 - 2215
- 全文大小：53K
- 年卷期：v.48,no.6(June 2000)
- ISSN：1520-5118
文摘
Bayluscide [the ethanolamine salt of niclosamide (NIC)] is a registered piscicide used in combinationwith 3-(trifluoromethyl)-4-nitrophenol (TFM) to control sea lamprey populations in streams tributaryto the Great Lakes. A high-performance liquid chromatography (HPLC) method was developed forthe determination of NIC residues in muscle fillet tissues of fish exposed to NIC and TFM duringsea lamprey control treatments. NIC was extracted from fortified channel catfish and rainbow troutfillet tissue with a series of acetone extractions and cleaned up on C18 solid-phase extractioncartridges. NIC concentrations were determined by HPLC with detection at 360 and 335 nm forrainbow trout and catfish, respectively. Recovery of NIC from rainbow trout (n = 7) fortified at 0.04
g/g was 77 ± 6.5% and from channel catfish (n = 7) fortified at 0.02 g/g was 113 ± 11%. NICdetection limit was 0.0107 g/g for rainbow trout and 0.0063 g/g for catfish. Percent recovery ofincurred radioactive residues by this method from catfish exposed to [14C]NIC was 89.3 ± 4.1%.Percent recoveries of NIC from fortified storage stability tissue samples for rainbow trout (n = 3)analyzed at 5 and 7.5 month periods were 78 ± 5.1 and 68 ± 2.4%, respectively. Percent recoveriesof NIC from fortified storage stability tissue samples for channel catfish (n = 3) analyzed at 5 and7.5 month periods were 88 ± 13 and 76 ± 21%, respectively.Keywords: Niclosamide; fillet residues; channel catfish; rainbow trout; HPLC
g/g was 77 ± 6.5% and from channel catfish (n = 7) fortified at 0.02 g/g was 113 ± 11%. NICdetection limit was 0.0107 g/g for rainbow trout and 0.0063 g/g for catfish. Percent recovery ofincurred radioactive residues by this method from catfish exposed to [14C]NIC was 89.3 ± 4.1%.Percent recoveries of NIC from fortified storage stability tissue samples for rainbow trout (n = 3)analyzed at 5 and 7.5 month periods were 78 ± 5.1 and 68 ± 2.4%, respectively. Percent recoveriesof NIC from fortified storage stability tissue samples for channel catfish (n = 3) analyzed at 5 and7.5 month periods were 88 ± 13 and 76 ± 21%, respectively.Keywords: Niclosamide; fillet residues; channel catfish; rainbow trout; HPLC