pH-Switchable Fluorescent Probe for Spatially-Confined Visualization of Intracellular Hydrogen Peroxide

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• 作者：Jun Liu ; Jing Ren ; Xiaojia Bao ; Wei Gao ; Chuanliu Wu ; Yibing Zhao
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 7, 2016
• 年：2016
• 卷：88
• 期：11
• 页码：5865-5870
• 全文大小：396K
• 年卷期：0
• ISSN：1520-6882

文摘

Intracellular H₂O₂ plays an important role in regulating a variety of cellular functions. Fluorescent probes that can make response to intracellular levels of H₂O₂ would provide valuable tools for revealing the functions of H₂O₂ in living organisms. However, traditional pH-insensitive probes and lysosome-targetable probes can only provide spatially nonspecific visualization of intracellular H₂O₂ and specific sensing of lysosomal H₂O₂, respectively. In this work, we developed a H₂O₂-responsive and pH-switchable fluorescent probe (HP-L1) which can make response sequentially to intracellular H₂O₂ and lysosomal pH. The fluorescent probe is comprised of a H₂O₂-responsive boronate moiety and a pH-switchable spirobenzopyran fluorophore. When the probe was applied for intracellular H₂O₂ sensing, only fluorescent emission from lysosomes is visible, and the fluorescence from other regions is not able to be obviously detected, which is due to the pH-switchable property of the spirobenzopyran fluorophore. Thus, the developed fluorescence probe enables the spatially confined (i.e., lysosome-specific) visualization of the intracellular H₂O₂. We envisioned that this kind of fluorescent probe (or the proposed sensing strategy) would allow the visualization of the overall levels of intracellular H₂O₂ without interferences of possible fluorescent signals from other sources (e.g., dyes for cellular staining and multiplex analysis).