Polymer Multilayers with pH-Triggered Release of Antibacterial Agents

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• 作者：Svetlana Pavlukhina ; Yiming Lu ; Altida Patimetha ; Matthew Libera ; Svetlana Sukhishvili
• 刊名：Biomacromolecules
• 出版年：2010
• 出版时间：December 13, 2010
• 年：2010
• 卷：11
• 期：12
• 页码：3448-3456
• 全文大小：486K
• 年卷期：v.11,no.12(December 13, 2010)
• ISSN：1526-4602

文摘

We report on the layer-by-layer design principles of poly(methacrylic acid) (PMAA) ultrathin hydrogel coatings that release antimicrobial agents (AmAs) in response to pH variations. The studied AmAs include gentamicin and an antibacterial cationic peptide L5. Adipic acid dihydrazide (AADH) is a cross-linker which, relative to ethylenediamine (EDA), increases the hydrogel hydrophobicity and introduces centers for hydrogen bonding to AmAs. AmA retention in AADH-cross-linked hydrogels in high-salt solutions was enhanced while AmA release at low pH was suppressed. L5 retains its antibacterial activity toward planktonic Staphylococcus epidermidis after release from PMAA hydrogels in response to pH decreases in the surrounding medium due to bacterial growth. Staphylococcus epidermidis adhesion and colonization was almost completely inhibited by L5 loading of hydrogels. The AmA-releasing and AmA-retaining properties of these hydrogel coatings provide new opportunities to study the fundamental mechanisms of AmA-coating−bacteria interactions and develop a new class of clinically relevant antibacterial coatings for medical devices.