A Novel Fluorescent Conjugate Applicable To Visualize the Translocation of Glucose鈥揊ipronil
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- 作者：Jie Wang ; Zhiwei Lei ; Yingjie Wen ; Genlin Mao ; Hanxiang Wu ; Hanhong Xu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：September 3, 2014
- 年：2014
- 卷：62
- 期：35
- 页码：8791-8798
- 全文大小：407K
- ISSN：1520-5118
文摘
The ability to visualize the movement of glycosyl insecticides contributes to learning their translocation and distribution in plants. In our present work, a novel fluorescent glucose鈥揻ipronil conjugate N-[3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl)sulfinyl]-1H-pyrazol-5-yl]-1-(2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-尾-d-glucopyranosyl)-1H-1,2,3-triazole-4-methanamine (2-NBDGTF), was obtained via click chemistry. Disk uptake experiments showed that an active carrier-mediated system was involved in the 2-NBDGTF uptake process. Meanwhile, 2-NBDGTF exhibited comparable phloem mobility with GTF in castor bean seedlings. Visualization of 2-NBDGTF uptake and transport experiment showed that this fluorescent glucose鈥揻ipronil conjugate could be loaded into sieve tubes after transiting through epidermal cells and mesophyll cells and then translocate from cotyledon to hypocotyl via phloem in castor bean seedlings. The results above determined that it is a promising fluorescence tagging approach for revealing the activities of glycosyl insecticides and 2-NBDGTF is a reasonable and feasible fluorescent surrogate of GTF for tracing the distribution of glucose鈥揻ipronil conjugates.