Simultaneous Fluorescence Sensing of Cys and GSH from Different Emission Channels
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- 作者：Jing Liu ; Yuan-Qiang Sun ; Yingying Huo ; Hongxing Zhang ; Linfang Wang ; Pei Zhang ; Dan Song ; Yawei Shi ; Wei Guo
- 刊名：Journal of the American Chemical Society
- 出版年：2014
- 出版时间：January 15, 2014
- 年：2014
- 卷：136
- 期：2
- 页码：574-577
- 全文大小：330K
- ISSN：1520-5126
文摘
A chlorinated coumarin-hemicyanine dye with three potential reaction sites was exploited as fluorescent probe for biothiols. The Cys-induced substitution鈥搑earrangement鈥揷yclization, Hcy-induced substitution鈥搑earrangement, and GSH-induced substitution鈥揷yclizatioin cascades lead to the corresponding amino-coumarin, amino-coumarin-hemicyanine, thiol-coumarin with distinct photophysical properties, enabling Cys and GSH to be selectively detected from different emission channels at two different excitation wavelengths.