Fluorescent Probe-Based Lateral Flow Assay for Multiplex Nucleic Acid Detection
详细信息 查看全文
- 作者：Ye Xu ; Yinghua Liu ; Yan Wu ; Xiaohu Xia ; Yiqun Liao ; Qingge Li
- 刊名：Analytical Chemistry
- 出版年：2014
- 出版时间：June 17, 2014
- 年：2014
- 卷：86
- 期：12
- 页码：5611-5614
- 全文大小：235K
- 年卷期：v.86,no.12(June 17, 2014)
- ISSN：1520-6882
文摘
Here we report a rapid, low cost, and disposable dipstick-type DNA biosensor that enables multiplex detection in a single assay. The fluorescent probes labeled with different fluorophores were introduced into the lateral flow nucleic acid testing system. In combination with multiple immobilized probes arranged in an array formant on the membrane, a dual-color fluorescent lateral flow DNA biosensor was developed using a portable fluorescence reader. Up to 13 human papillomavirus types could be detected simultaneously by a single-step operation in less than 30 min after linear-after-the-exponential (LATE)-PCR. The sensitivity was determined to be 10鈥?02 copies plasmid DNA/渭L. The specificity study showed no cross-reactivity among the 31 different common HPV types. In the clinical validation, 95.3% overall agreement showed very good potential for this method in the clinical application when compared to a commercial kit.