Characterization of an Ethanol-Inducible Promoter System in Catharanthus roseus Hairy Roots

详细信息    查看全文

• 作者：Christie A. M. Peebles ; Susan I. Gibson ; Jacqueline V. Shanks ; Ka-Yiu San
• 刊名：Biotechnology Progress
• 出版年：2007
• 出版时间：October 2007
• 年：2007
• 卷：23
• 期：5
• 页码：1258 - 1260
• 全文大小：70K
• 年卷期：v.23,no.5(October 2007)
• ISSN：1520-6033

文摘

Efforts to engineer Catharanthus roseus hairy roots to produce commercially significant amountsof valuable compounds, such as the terpenoid indole alkaloids vinblastine and vincristine, requirethe development of tools to study the effects of overexpressing key metabolic and regulatorygenes. The use of inducible promoters allows researchers to control the timing and level ofexpression of genes of interest. In addition, use of inducible promoters allows researchers touse a single transgenic line as both the control and experimental line, minimizing the problemsassociated with clonal variation. We have previously characterized the use of a glucocorticoid-inducible promoter system to study the effects of gene overexpression within the terpenoid indolealkaloid pathway on metabolite production. Here the feasibility of using an ethanol-induciblepromoter within C. roseus hairy roots is reported. This ethanol-inducible promoter is highlysensitive to ethanol concentration with a concentration of 0.005% ethanol causing a 6-fold increasein CAT reporter activity after 24 h of induction. The ethanol-inducible CAT activity increased24-fold over a 72-h induction period with 0.5% ethanol.